A fluid-fluid level is a fairly specific sign for the entities listed below. Being aware of the differential is important since the underlying etiology could be either benign or very malignant. Other features and the clinical context can help narrow down the diagnosis further. The mnemonic here is not the greatest, but to remember the diagnoses, think of the DNA bases:
ACGT:
| Disease | Notes |
| A | Aneursymal Bone Cyst | Benign; 80% < age 20 |
| C | Chondroblastoma | Age<20 |
| G | Giant Cell Tumor | Seen only after physeal closure (age>20) |
| T | Telangiectatic Osteosarcoma | Most common location: Femoral metaphysis |
The age of the patient is an important factor to consider, as the GCT only occurs after physeal closure.
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